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| 产品名称 | β-actin Rabbit Polyclonal Antibody | 反应种属 | H,M,R,Mk,Ch,Hm,Sh,Xenopus |
| 应用 | WB,IHC,IF | ||
| 配方 | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| 别称 | N/A | ||
| 储存 | Store at -20°C. Stable for one year from the date of shipment. | ||
| 推荐稀释比 | WB 1:3,000 IHC 1:200 IF 1:200 | ||
| 特异性 | Antibody can detect endogenous β-actin proteins. | ||
| 背景信息 | β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues. | ||
| 分子量 | 43KD | 同型 | IgG |
| GeneID | 60 | 理论分子量 | 43KD |
| SWISS | P60709 | 宿主物种 | Rabbit |
| 免疫原 | Recombinant Protein | 克隆性 | Polyclonal |
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Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000.
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IHC staining of Human ovary tissue with β-Actin pAb diluted at 1:200.