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| 产品名称 | HSP70 Mouse Monoclonal Antibody (3G10) | 反应种属 | H,M,R,Pg |
| 应用 | WB,IHC,IF | ||
| 配方 | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| 别称 | N/A | ||
| 储存 | Store at -20°C. Stable for one year from the date of shipment. | ||
| 推荐稀释比 | WB 1:1,000-2,000 IHC 1:200-500 IF 1:100-200 | ||
| 特异性 | Antibody can detect endogenous HSP70 proteins. | ||
| 背景信息 | The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding,and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity,such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes,the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues,and interacts with them. This spontaneous interaction is reversible,and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However,the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70,increasing its normally slow rate of ATP hydrolysis. | ||
| 分子量 | 70KD | 同型 | IgG1 |
| GeneID | 3303 | 理论分子量 | 70KD |
| SWISS | P08107 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2,000.
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Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using HSP70 Mouse mAb diluted at 1:500.