当前位置：
说明书下载产品属性/ Product Details
| 产品名称 | Human AP36(Apelin 36) ELISA Kit | 反应种属 | Human |
| Uniprot ID | Q9ULZ1(42~77aa) | 别称 | AP36 |
| 检测方法 | Competitive | 灵敏度 | 28.13 pg/mL |
| 标准品 | 3000pg/mL | 检测范围 | 46.88-3000pg/mL |
| 样品类型 | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL | ||
| 检测时间 | 1.5H | 研究领域 | Metabolic pathway;Nutrition metabolism; |
| 测试原理 | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human AP36. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human AP36. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | OD |
|---|---|
| 3000.00 | 0.243 |
| 1500.00 | 0.365 |
| 750.00 | 0.543 |
| 375.00 | 0.827 |
| 187.50 | 1.136 |
| 93.75 | 1.504 |
| 46.88 | 1.777 |
| 0.00 | 2.525 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(pg/mL) | 17.65 | 659.05 | 811.64 | 119.45 | 614.53 | 1443.15 |
| Standard deviation | 1.68 | 32.33 | 67.14 | 2.11 | 22.85 | 59.69 |
| C V (%) | 5.88 | 2.77 | 5.06 | 6.34 | 4.42 | 6.75 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 82-95 | 88 |
| EDTA plasma (n=8) | 80-95 | 87 |
| Cell culture media (n=8) | 86-99 | 92 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 93-101 | 93-102 | 82-95 |
| Average (%) | 100 | 94 | 95 | |
| 1:4 | Range (%) | 97-103 | 93-102 | 89-101 |
| Average (%) | 98 | 98 | 99 | |
| 1:8 | Range (%) | 91-99 | 81-96 | 87-98 |
| Average (%) | 91 | 91 | 91 | |
| 1:16 | Range (%) | 90-98 | 93-106 | 92-104 |
| Average (%) | 94 | 98 | 97 |
检测流程/ Assay Procedures
