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说明书下载产品属性/ Product Details
| 产品名称 | Human AP13(Apelin 13) ELISA Kit | 反应种属 | Human |
| Uniprot ID | Q9ULZ1(65~77aa) | 别称 | AP13 |
| 检测方法 | Competitive | 灵敏度 | 75 pg/mL |
| 标准品 | 8000pg/mL | 检测范围 | 125-8000pg/mL |
| 样品类型 | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL | ||
| 检测时间 | 1.5H | 研究领域 | Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism; |
| 测试原理 | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human AP13. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | OD |
|---|---|
| 8000.00 | 0.204 |
| 4000.00 | 0.361 |
| 2000.00 | 0.571 |
| 1000.00 | 0.835 |
| 500.00 | 1.119 |
| 250.00 | 1.419 |
| 125.00 | 1.753 |
| 0.00 | 2.549 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(pg/mL) | 340.83 | 1603.46 | 2861.56 | 499.11 | 1479.01 | 4348.05 |
| Standard deviation | 6.94 | 94.34 | 189.01 | 23.14 | 86.92 | 170.12 |
| C V (%) | 7.16 | 4.82 | 6.61 | 4.20 | 3.94 | 2.98 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 88-102 | 95 |
| EDTA plasma (n=8) | 80-97 | 88 |
| Cell culture media (n=8) | 83-95 | 89 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 86-95 | 80-91 | 91-97 |
| Average (%) | 88 | 80 | 95 | |
| 1:4 | Range (%) | 82-93 | 80-91 | 95-104 |
| Average (%) | 89 | 87 | 101 | |
| 1:8 | Range (%) | 97-106 | 86-92 | 97-105 |
| Average (%) | 103 | 90 | 104 | |
| 1:16 | Range (%) | 89-102 | 89-96 | 82-95 |
| Average (%) | 100 | 90 | 86 |
检测流程/ Assay Procedures
