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产品属性/ Product Details
| 产品名称 | Human Salusin α(Salusin Alpha) ELISA Kit | 反应种属 | Human |
| Uniprot ID | Q8N2E6(214~241aa) | 别称 | Salusin-alpha |
| 检测方法 | Competitive | 灵敏度 | 46.88 pg/mL |
| 标准品 | 5000pg/mL | 检测范围 | 78.13-5000pg/mL |
| 样品类型 | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL | ||
| 检测时间 | 1.5H | 研究领域 | Endocrinology; |
| 测试原理 | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Salusin α. During the reaction, Human Salusin α in the sample or standard competes with a fixed amount of Human Salusin α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Salusin α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Salusin α in tested samples can be calculated by comparing the OD of the samples to the standard curve. | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | OD |
|---|---|
| 5000.00 | 0.161 |
| 2500.00 | 0.299 |
| 1250.00 | 0.518 |
| 625.00 | 0.849 |
| 312.50 | 1.270 |
| 156.25 | 1.727 |
| 78.13 | 2.036 |
| 0.00 | 2.516 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(pg/mL) | 240.16 | 1111.48 | 1281.73 | 221.47 | 1000.79 | 1602.79 |
| Standard deviation | 8.12 | 34.94 | 86.12 | 4.55 | 36.64 | 86.34 |
| C V (%) | 6.84 | 4.34 | 7.03 | 5.27 | 4.04 | 2.97 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 83-97 | 90 |
| EDTA plasma (n=8) | 86-99 | 92 |
| Cell culture media (n=8) | 89-103 | 96 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 91-98 | 80-97 | 82-93 |
| Average (%) | 95 | 93 | 84 | |
| 1:4 | Range (%) | 83-101 | 80-97 | 95-104 |
| Average (%) | 87 | 96 | 98 | |
| 1:8 | Range (%) | 87-98 | 81-92 | 82-96 |
| Average (%) | 88 | 91 | 94 | |
| 1:16 | Range (%) | 95-102 | 87-103 | 92-105 |
| Average (%) | 97 | 98 | 105 |