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产品属性/ Product Details
| 产品名称 | Human Salusin β(Salusin Beta) ELISA Kit | 反应种属 | Human |
| Uniprot ID | N/A | 别称 | Salusin-beta |
| 检测方法 | Competitive | 灵敏度 | 18.75 pg/mL |
| 标准品 | 2000pg/mL | 检测范围 | 31.25-2000pg/mL |
| 样品类型 | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL | ||
| 检测时间 | 1.5H | 研究领域 | Neuro science; |
| 测试原理 | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Salusin β. During the reaction, Human Salusin β in the sample or standard competes with a fixed amount of Human Salusin β on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Salusin β. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Salusin β in tested samples can be calculated by comparing the OD of the samples to the standard curve. | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | OD |
|---|---|
| 2000.00 | 0.177 |
| 1000.00 | 0.313 |
| 500.00 | 0.528 |
| 250.00 | 0.862 |
| 125.00 | 1.290 |
| 62.50 | 1.682 |
| 31.25 | 2.058 |
| 0.00 | 2.498 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(pg/mL) | 79.14 | 465.10 | 736.00 | 114.74 | 275.45 | 923.02 |
| Standard deviation | 2.04 | 23.95 | 44.74 | 2.82 | 15.47 | 50.46 |
| C V (%) | 2.73 | 4.80 | 4.93 | 4.05 | 5.63 | 2.75 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 80-92 | 85 |
| EDTA plasma (n=8) | 84-106 | 100 |
| Cell culture media (n=8) | 83-95 | 89 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 85-98 | 82-96 | 87-96 |
| Average (%) | 95 | 84 | 94 | |
| 1:4 | Range (%) | 95-104 | 82-96 | 92-105 |
| Average (%) | 100 | 95 | 102 | |
| 1:8 | Range (%) | 93-102 | 86-98 | 85-102 |
| Average (%) | 100 | 86 | 101 | |
| 1:16 | Range (%) | 83-97 | 93-101 | 80-101 |
| Average (%) | 89 | 97 | 86 |