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货号:FS1117H
FS-人5 核苷酸酶(5-NT)酶联免疫吸附测定试剂盒
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规格:

48 T

96 T

价格:

¥2100

¥3000

货期: 现货

产品属性/ Product Details
产品名称 FS-Human 5-NT(5-Nucleotidase) ELISA Kit 反应种属 Human
Uniprot ID P21589 别称 5' nucleotidase (CD73), 5'-NT, 5-NT, 5'-Nucleotidase, 5'-nucleotidase, ecto (CD73), CD73 antigen, CD73, E5NT, EC 3.1.3.5, Ecto-5'-nucleotidase, eN, eNT, NT, NT55'-nucleotidase, NT5E, NTE, Purine 5-Prime-Nucleotidase
检测方法 Sandwich 灵敏度 2.86 pg/mL
标准品 2000pg/mL 检测范围 31.25-2000pg/mL
样品类型 Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
检测时间 1.5H 研究领域 Signal transduction;Tumor immunity;Infection immunity;
测试原理 This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human 5-NT are added to the micro ELISA plate wells. Human 5-NT in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human 5-NT. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human 5-NT. The concentration of Human 5-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

(pg/mL) OD Corrected
2000.00 2.413 2.355
1000.00 1.415 1.357
500.00 0.850 0.792
250.00 0.494 0.436
125.00 0.291 0.233
62.50 0.179 0.121
31.25 0.112 0.054
0.00 0.058 0.000
精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 1723.734 977.440 889.308 450.374 444.014 373.350
Standard deviation 153.730 90.770 32.417 9.370 11.193 10.157
C V (%) 10.545 6.408 10.217 6.444 10.177 9.176
回收率

分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 90-110 96
EDTA plasma (n=8) 92-110 110
Cell culture media (n=8) 92-110 107
线性

分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 90-106 94-104 91-108
Average (%) 102 99 93
1:4 Range (%) 92-109 93-103 93-110
Average (%) 96 105 108
1:8 Range (%) 94-106 93-112 92-114
Average (%) 103 92 94
1:16 Range (%) 92-113 92-115 94-98
Average (%) 111 98 97