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产品属性/ Product Details
| 产品名称 | FS-Human PGA/PGⅠ( Pepsinogen A) ELISA Kit | 反应种属 | Human |
| Uniprot ID | P0DJD9 | 别称 | EC 3.4.23, EC 3.4.23.1, FLJ58952, FLJ77962, pepsin A, pepsinogen 4, group I (pepsinogen A), Pepsinogen A, pepsinogen A4, PGA3, PGA5, PGI, Pepsinogen I |
| 检测方法 | Sandwich | 灵敏度 | 0.23 ng/mL |
| 标准品 | 25ng/mL | 检测范围 | 0.39-25ng/mL |
| 样品类型 | Serum, Plasma；Sample Volume=50μL | ||
| 检测时间 | 1.5H | 研究领域 | Infection immunity;Immune molecule;Autoimmunity; |
| 测试原理 | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PGA/PGⅠ. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PGA/PGⅠ are added to the micro ELISA plate wells. Human PGA/PGⅠ in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PGA/PGⅠ. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human PGA/PGⅠ. The concentration of Human PGA/PGⅠ in the samples is then determined by comparing the OD of the samples to the standard curve. | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (ng/mL) | OD | Corrected |
|---|---|---|
| 25.00 | 3.269 | 3.266 |
| 12.50 | 1.966 | 1.963 |
| 6.25 | 0.822 | 0.819 |
| 3.13 | 0.442 | 0.439 |
| 1.56 | 0.198 | 0.195 |
| 0.78 | 0.081 | 0.078 |
| 0.39 | 0.045 | 0.042 |
| 0.00 | 0.003 | 0.000 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(ng/mL) | 11.709 | 2.417 | 18.344 | 15.726 | 7.767 | 17.646 |
| Standard deviation | 0.100 | 10.184 | 12.459 | 14.631 | 10.914 | 13.119 |
| C V (%) | 9.060 | 5.489 | 0.400 | 1.222 | 2.883 | 6.994 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 87-115 | 97 |
| EDTA plasma (n=8) | 96-103 | 101 |
| Cell culture media (n=8) | 89-106 | 102 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 89-107 | 90-107 | 85-111 |
| Average (%) | 102 | 95 | 102 | |
| 1:4 | Range (%) | 91-110 | 89-108 | 98-115 |
| Average (%) | 109 | 106 | 105 | |
| 1:8 | Range (%) | 85-113 | 99-106 | 92-106 |
| Average (%) | 102 | 103 | 95 | |
| 1:16 | Range (%) | 100-114 | 95-117 | 94-118 |
| Average (%) | 111 | 96 | 104 |