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货号:FS1739H
FS-人干细胞因子(SCF)酶联免疫吸附测定试剂盒
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规格:

48 T

96 T

价格:

¥2100

¥3000

货期: 现货

产品属性/ Product Details
产品名称 FS-Human SCF(Stem Cell Factor) ELISA Kit 反应种属 Human
Uniprot ID P21583 别称 c-kit Ligand, DCUA, DFNA69, DKFZp686F2250, familial progressive hyperpigmentation 2, FPH2, FPHH, KIT ligand, Kitl, KITLG, KL-1, Mast cell growth factor, MGF, MGFSHEP7, SCF, SCFStem cell factor, SFc-Kit ligand, SHEP7, SLF, steel factor
检测方法 Sandwich 灵敏度 3.76 pg/mL
标准品 2000pg/mL 检测范围 31.25-2000pg/mL
样品类型 Serum, Plasma;Sample Volume=50μL
检测时间 1.5H 研究领域 Signal transduction;
测试原理 This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCF. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human SCF are added to the micro ELISA plate wells. Human SCF in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human SCF. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human SCF. The concentration of Human SCF in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

(pg/mL) OD Corrected
2000.00 2.928 2.904
1000.00 1.739 1.715
500.00 0.939 0.915
250.00 0.523 0.499
125.00 0.278 0.254
62.50 0.156 0.132
31.25 0.091 0.067
0.00 0.024 0.000
精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 1199.007 1875.491 369.613 893.014 259.799 647.123
Standard deviation 4.960 14.609 1.996 1.757 12.592 11.325
C V (%) 4.615 1.615 6.571 6.526 5.478 0.675
回收率

分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 93-99 99
EDTA plasma (n=8) 96-107 98
Cell culture media (n=8) 84-103 86
线性

分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-95 98-108 93-110
Average (%) 90 101 108
1:4 Range (%) 98-107 86-115 85-109
Average (%) 99 101 91
1:8 Range (%) 95-114 88-116 91-115
Average (%) 99 96 93
1:16 Range (%) 96-114 99-105 83-112
Average (%) 101 99 104