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货号:FS1305H
FS-人天青杀素1(AZU1)酶联免疫吸附测定试剂盒
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规格:

48 T

96 T

价格:

¥2100

¥3000

货期: 现货

产品属性/ Product Details
产品名称 FS-Human HBP/AZU1(Azurocidin 1) ELISA Kit 反应种属 Human
Uniprot ID P20160 别称 AZAMP, AZU1, CAP37, HBP, HUMAZUR, NAZC, Cationic Antimicrobial Protein CAP37, Heparin Binding Protein
检测方法 Sandwich 灵敏度 22.30 pg/mL
标准品 4000pg/mL 检测范围 62.5-4000pg/mL
样品类型 Serum, Plasma;Sample Volume=50μL
检测时间 1.5H 研究领域 Cytokine;Hematology;
测试原理 This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HBP/AZU1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human HBP/AZU1 are added to the micro ELISA plate wells. Human HBP/AZU1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human HBP/AZU1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human HBP/AZU1. The concentration of Human HBP/AZU1 in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

(pg/mL) OD Corrected
4000.00 2.567 2.541
2000.00 1.344 1.318
1000.00 0.710 0.684
500.00 0.382 0.356
250.00 0.197 0.171
125.00 0.112 0.086
62.50 0.079 0.053
0.00 0.026 0.000
精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 3750.722 309.239 2850.580 1333.807 159.975 3409.779
Standard deviation 1.858 5.879 7.503 12.796 11.893 3.348
C V (%) 6.075 4.826 8.571 3.366 2.798 4.405
回收率

分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 91-110 107
EDTA plasma (n=8) 87-100 91
Cell culture media (n=8) 85-105 99
线性

分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-107 83-113 97-113
Average (%) 90 106 103
1:4 Range (%) 96-106 84-112 95-108
Average (%) 106 107 105
1:8 Range (%) 95-109 99-107 89-118
Average (%) 101 104 103
1:16 Range (%) 88-115 99-118 100-106
Average (%) 112 109 106