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货号:FS1094M
FS-小鼠基质金属蛋白酶抑制因子1(TIMP-1)酶联免疫吸附测定试剂盒
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规格:

48 T

96 T

价格:

¥2100

¥3000

货期: 现货

产品属性/ Product Details
产品名称 FS-Mouse TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit 反应种属 Mouse
Uniprot ID P12032 别称 CLGI, Collagenase inhibitor, collagenase inhibitor), EPATIMP-1, EPO, erythroid potentiating activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, metalloproteinase inhibitor 1, TIMP metallopeptidase inhibitor 1, TIMP1, TIMP-1, TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, Tissue inhibitor of metalloproteinases 1
检测方法 Sandwich 灵敏度 25.41 pg/mL
标准品 5000pg/mL 检测范围 78.13-5000pg/mL
样品类型 Serum, Plasma;Sample Volume=50μL
检测时间 1.5H 研究领域 Infection immunity;Hematology;
测试原理 This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse TIMP-1 are added to the micro ELISA plate wells. Mouse TIMP-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse TIMP-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse TIMP-1. The concentration of Mouse TIMP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

(pg/mL) OD Corrected
5000.00 2.975 2.958
2500.00 2.028 2.011
1250.00 1.167 1.150
625.00 0.678 0.661
312.50 0.371 0.354
156.25 0.194 0.177
78.13 0.109 0.092
0.00 0.017 0.000
精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 3178.784 1789.090 2109.269 2735.440 4037.058 214.725
Standard deviation 8.828 0.431 8.324 7.047 4.785 14.400
C V (%) 3.591 7.885 7.812 7.718 9.133 7.371
回收率

分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 93-113 101
EDTA plasma (n=8) 94-101 97
Cell culture media (n=8) 83-111 92
线性

分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-110 91-112 84-110
Average (%) 104 100 99
1:4 Range (%) 89-111 102-107 96-107
Average (%) 98 107 106
1:8 Range (%) 98-108 95-113 83-117
Average (%) 104 110 114
1:16 Range (%) 83-115 102-110 90-112
Average (%) 104 106 102