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产品属性/ Product Details
| 产品名称 | FS-Human TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit | 反应种属 | Human |
| Uniprot ID | P01033 | 别称 | CLGI, Collagenase inhibitor, collagenase inhibitor), EPATIMP-1, EPO, erythroid potentiating activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, metalloproteinase inhibitor 1, TIMP metallopeptidase inhibitor 1, TIMP1, TIMP-1, TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, Tissue inhibitor of metalloproteinases 1 |
| 检测方法 | Sandwich | 灵敏度 | 31.25 pg/mL |
| 标准品 | 5000pg/mL | 检测范围 | 78.13-5000pg/mL |
| 样品类型 | Serum, Plasma；Sample Volume=50μL | ||
| 检测时间 | 1.5H | 研究领域 | Cytokine;Immune molecule;Hematology; |
| 测试原理 | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human TIMP-1 are added to the micro ELISA plate wells. Human TIMP-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human TIMP-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human TIMP-1. The concentration of Human TIMP-1 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | OD | Corrected |
|---|---|---|
| 5000.00 | 3.418 | 3.402 |
| 2500.00 | 2.491 | 2.475 |
| 1250.00 | 1.650 | 1.634 |
| 625.00 | 0.862 | 0.846 |
| 312.50 | 0.457 | 0.441 |
| 156.25 | 0.250 | 0.234 |
| 78.13 | 0.133 | 0.117 |
| 0.00 | 0.016 | 0.000 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(pg/mL) | 3647.750 | 3779.602 | 861.049 | 2228.664 | 3045.702 | 1404.329 |
| Standard deviation | 0.957 | 6.466 | 2.626 | 8.073 | 10.684 | 9.628 |
| C V (%) | 5.595 | 0.862 | 8.540 | 7.609 | 6.813 | 3.860 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 91-107 | 104 |
| EDTA plasma (n=8) | 86-106 | 89 |
| Cell culture media (n=8) | 82-104 | 85 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 94-96 | 94-104 | 84-116 |
| Average (%) | 96 | 99 | 105 | |
| 1:4 | Range (%) | 97-108 | 93-112 | 89-108 |
| Average (%) | 97 | 95 | 105 | |
| 1:8 | Range (%) | 99-118 | 100-105 | 89-111 |
| Average (%) | 116 | 102 | 103 | |
| 1:16 | Range (%) | 87-117 | 100-108 | 91-107 |
| Average (%) | 113 | 100 | 105 |