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货号:FS1049R
FS-大鼠白介素1受体Ⅱ(IL-1R2)酶联免疫吸附测定试剂盒
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规格:

48 T

96 T

价格:

¥2100

¥3000

货期: 现货

产品属性/ Product Details
产品名称 FS-Rat IL-1R2(Interleukin 1 Receptor Type Ⅱ) ELISA Kit 反应种属 Rat
Uniprot ID P43303 别称 beta, CD121b antigen, CD121b, IL-1 R beta, IL-1 RII, IL1R2, IL1RBCD121 antigen-like family member B, IL-1R-beta, IL1RII, IL-1RII, IL-1RT2, IL-1RT-2, interleukin 1 receptor, type II, Interleukin-1 receptor beta, MGC47725
检测方法 Sandwich 灵敏度 5.57 pg/mL
标准品 1000pg/mL 检测范围 15.63-1000pg/mL
样品类型 Serum, Plasma;Sample Volume=50μL
检测时间 1.5H 研究领域 Enzyme & Kinase;Tumor immunity;Infection immunity;Hepatology;
测试原理 This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells. Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IL-1R2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat IL-1R2. The concentration of Rat IL-1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.
技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

(pg/mL) OD Corrected
1000.00 2.007 1.985
500.00 1.146 1.124
250.00 0.668 0.646
125.00 0.338 0.316
62.50 0.177 0.155
31.25 0.079 0.057
15.63 0.048 0.026
0.00 0.022 0.000
精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 127.054 881.927 387.867 472.957 79.163 658.324
Standard deviation 14.951 11.492 4.879 1.423 12.194 6.799
C V (%) 9.755 4.163 9.766 4.754 9.599 5.714
回收率

分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 89-96 90
EDTA plasma (n=8) 91-106 104
Cell culture media (n=8) 83-104 83
线性

分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 92-101 91-118 84-111
Average (%) 101 118 94
1:4 Range (%) 87-104 91-108 90-110
Average (%) 95 103 103
1:8 Range (%) 103-115 98-110 92-113
Average (%) 103 100 94
1:16 Range (%) 101-117 101-116 92-115
Average (%) 110 109 102