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| 产品名称 | His-Tag Mouse Monoclonal Antibody(4E6) | 反应种属 | N/A |
| 应用 | WB,IF,IP | ||
| 配方 | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| 别称 | N/A | ||
| 储存 | Store at -20°C. Stable for one year from the date of shipment. | ||
| 推荐稀释比 | WB 1:3,000 IF 1:1,000 IP 1:200 | ||
| 特异性 | Antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins. | ||
| 背景信息 | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. | ||
| 分子量 | N/A | 同型 | IgG1 |
| GeneID | N/A | 理论分子量 | N/A |
| SWISS | N/A | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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2ug His fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
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IF analysis of 293 cells transfected with a His-tag protein, using anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His)