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| 产品名称 | NSE Mouse Monoclonal Antibody(13E2) | 反应种属 | H,M,R |
| 应用 | WB,IHC | ||
| 配方 | 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. | ||
| 别称 | N/A | ||
| 储存 | Store at -20°C. Stable for one year from the date of shipment. | ||
| 推荐稀释比 | WB 1:2,000 IHC 1:200 | ||
| 特异性 | Antibody can detect endogenous NSE proteins. | ||
| 背景信息 | Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals,enolase molecules are dimers composed of three distinct subunits (alpha,beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons,in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. | ||
| 分子量 | 47KD | 同型 | IgG1 |
| GeneID | 2026 | 理论分子量 | 47KD |
| SWISS | P09104 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of 1)Hela, 2)Jurkat, 3)293T cell lysates with NSE mAb diluted at 1:3,000.
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IHC staining of Human small cell carcinoma of lung tissue with NSEmouse mAb diluted at 1:200.