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说明书下载产品属性/ Product Details
| 产品名称 | Rat IL-6(Interleukin 6) ELISA Kit | 反应种属 | Rat |
| Uniprot ID | P20607 | 别称 | B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A |
| 检测方法 | 双抗体夹心法 | 灵敏度 | 28.77 pg/mL |
| 标准品 | 2000pg/mL | 检测范围 | 31.25- 2000pg/mL |
| 样品类型 | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL | ||
| 检测时间 | 3.5小时 | 研究领域 | Tumor immunity;Endocrinology;Hormone metabolism; |
| 测试原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-6抗体包被于酶标板上，实验时样品或标准品中的大鼠IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗大鼠IL-6抗体和辣根过氧化物酶标记的亲和素。抗大鼠IL-6抗体与结合在包被抗体上的大鼠IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中大鼠IL-6的浓度。 | ||
技术数据/ Technical Data
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | OD | Corrected |
|---|---|---|
| 2000.00 | 2.392 | 2.340 |
| 1000.00 | 1.745 | 1.693 |
| 500.00 | 0.873 | 0.821 |
| 250.00 | 0.473 | 0.421 |
| 125.00 | 0.264 | 0.212 |
| 62.50 | 0.131 | 0.079 |
| 31.25 | 0.095 | 0.043 |
| 0.00 | 0.052 | 0.000 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(pg/mL) | 13.51 | 191.69 | 357.43 | 14.35 | 164.67 | 355.13 |
| Standard deviation | 1.19 | 7.21 | 17.16 | 1.00 | 8.04 | 10.81 |
| C V (%) | 7.40 | 5.55 | 4.79 | 7.54 | 3.80 | 6.16 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range(%) | Average Recovery(%) |
|---|---|---|
| Serum (n=8) | 83-97 | 90 |
| EDTA plasma (n=8) | 87-95 | 91 |
| Cell culture media (n=8) | 81-95 | 88 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 93-102 | 86-96 | 87-96 |
| Average (%) | 96 | 95 | 92 | |
| 1:4 | Range (%) | 81-96 | 93-104 | 85-97 |
| Average (%) | 89 | 98 | 96 | |
| 1:8 | Range (%) | 93-106 | 89-97 | 85-94 |
| Average (%) | 96 | 93 | 88 | |
| 1:16 | Range (%) | 82-95 | 85-94 | 87-96 |
| Average (%) | 88 | 93 | 93 |
检测流程/ Assay Procedures
相关文献/ Citations(7)
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IF：8.3
IF：8.3
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Species:Rat
Sample Types:Rat serum and Tissue homogenate
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IF：3.2
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Species:Rat
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IF：2.8
IF：2.8
Journal:Molecular Biology Reports
Species:Rat
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